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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: J Neurosci Res. 2015 Feb 20;93(8):1215–1228. doi: 10.1002/jnr.23573

Fig 6. HDACi Tubacin increases microtubule acetylation, while HATis moderately decrease histone acetylation.

Fig 6

A and B: Western immunoblots of lysates from adult DRG neuronal cultures treated with DMSO, AA, CPTH2, TSA or Tubacin for 48 hrs were probed with acetylated tubulin antibody (6–11-b-1) and with alpha tubulin antibody. Blots were developed using the LiCOR Odyssey system. Densitometric ratio of acetylated tubulin was ratioed against densitometric ratio of total alpha tubulin. In lysates treated with AA, acetylated tubulin did not change compared to controls treated with DMSO. In lysates treated with CPTH2 and TSA, acetylated tubulin increased, but the increase was not significant. In lysates treated with Tubacin, acetylated tubulin increased compared with controls. C and D: Western immunoblots of lysates from adult DRG neuronal cultures were probed with acetylated histone H3 antibody and with total histone H3 antibody. Blots were developed using ECL and exposed to X-ray film. Densitometric ratio of acetylated histone H3 was ratioed against densitometric ratio of total histone H3. In lysates treated with AA and CPTH2, acetylated histone H3 decreased compared to controls treated with DMSO, but no significant difference was observed. In lysates treated with Tubacin and with TSA, acetylated histone H3 remained unchanged compared to controls. * p<0.05.