Table 2.

Validation of microarray results with quantitative PCR

Bacterial group *	Adult sample aHLT02 †		Adult sample aHLT05 †
	qPCR ‡	Array ‡	qPCR ‡	Array ‡
Faecalibacterium	11.7%±5.0%	11.0%±0.3%	4.9%±3.5%	9.1%±0.2%
Bifidobacterium	5.7%±2.2%	6.4%±0.3%	0.3%±0.2%	0.1%±<0.1%
Collinsella	1.0%±0.3%	0.7%±0.1%	0.1%±0.1%	0.3%±<0.1%

^*Primers were developed to cover most of the 16S rDNA sequences in the RDP database belonging to each group (Rigsbee, et al., 2011).

^†Numbers represent relative abundances of genomic DNA of each genus in the total sample; no 16S rRNA gene copy adjustment was applied to the normalized values.

^‡Data is shown as arithmetic mean ± standard error.