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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Antiviral Res. 2015 Jun 15;120:134–139. doi: 10.1016/j.antiviral.2015.06.006

Fig. 2. Cell-free luciferase reporter assay.

Fig. 2

PPMO were added to in vitro translation reactions containing RNA transcribed from reporter constructs that include PPMO target sequences upstream of and in-frame with firefly luciferase coding sequence. Reactions with a construct containing HP1 target sequence and treated with PPMO CP1 were used as a negative control. Luciferase activity in the presence of the various PPMO is graphed as the relative percentage of untreated control reactions, set as 100%. The average of three tests is shown and the error bars represent standard errors among the experiments. ** indicates significant differences from CP1 at corresponding concentrations (P < 0.01).