Figure 3.

Methylation density of miR-378 5’-flanking region identified in two normal controls, three hypomethylated and three methylated AML patients by bisulfite sequencing. Black lollipop: methylated CpG dinucleotide; Blank lollipop: unmethylated CpG dinucleotide. A-C: three hypomethylated samples, A: M2, B: M4a, C: M5b; D-F: three methylated samples, D: M3, E: M1, F: M5b; G-H: normal controls. The results of RQ-MSP and bisulfite-sequencing were significantly negative correlated (R=-0.829, P=0.042).