Quality control of MSCs in vitro. (A) Representative FACS analysis demonstrated positivity for MSC markers CD73, CD90, CD105, but negativity for CD11b, CD19, CD34, and CD45. The cells differentiated to (B) adipocytes, (C) chondrocytes, (D) osteoblasts. (E) After labeling with PKH26 the cells show strong red fluorescence detected by fluorescence microscopy. Scale bar, 50 μm.