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. 2015 May 1;8(5):4525–4534.

Figure 5.

Figure 5

Effect of miR-34c on Notch pathway. A. RT-qPCR analysis of the mRNA expression levels of Hes1 and Hey1 in different treated groups. Podocytes treated with pre-miR-34 were stimulated with HG for 48 h before being collected for analysis. N = 3, *P < 0.05 and **P < 0.01 vs. Normal; &P < 0.05 and &&P < 0.01 vs. Blank or Pre-miR Ctrl. B. Western blot analysis of the protein expression levels of NICD, Hes1 and Hey1 in different treated groups. C. Quantitative protein levels were normalized to β-actin using Image-Pro Plus 6.0 software. Statistical analysis was calculated by one-way ANOVA. N = 3, **P < 0.01 vs. Normal; &P < 0.05 and &&P < 0.01vs. Blank or Pre-miR Ctrl.