Analysis of surface CD80/CD86 by flow cytometry in siRNA interferenced macrophages infected with H. pylori. Macrophages interferenced with miR-155mimic, miR-155 inhibitors, then 106 cells were analyzed on FACS and obtained fluorescence intensities of CD80/CD86. Bar graphs show the mean fluorescence intensities (MFI) of CD80/CD86 (mean ± SE, 3 independent experiments), and statistical analysis was by SPSS 16.0, with asterisks indicating the pairs of values compared for which significant differences were observed (*P≤0.05).