Figure 4.

The loss of function of miR-182 when over-expression of DNMT3a in cervical cancer cell line. (A) and (B)The expression analysis of DNMT3a both in mRNA (A) and protein (B) levels in cervical cell treated with indicated dose of pcDNA3-DNMT3a by real time PCR and Western blotting. Empty vector was used as negative control in this experiment. Data were presented as mean ± SD, *P < 0.05, **P < 0.01. (C-E) The apoptosis analysis of cervical cell treated simultaneously without (C) or with miR-182 (D) and miR-182 plus pcDNA3-DNMT3a (E).