Figure 2.

Overexpression of miR-302a enhances ovarian cancer cells proliferation and promotes the cell cycle. A. The relative level of miR-302a expressed in OC cells after the transfection with miR-302a or control vector. B. The cell independent growth activity in vitro was assessed by colony formation assay. OC cells were transfected with miR-302a or control vector. The colony formation assay was shown. C. OC cells were transfected with miR-302a or control vector, and then seeded in 6-well plates. Colonies were counted only if they contained more than 50 cells, and the number of colonies was counted from the 6th day after seeding. The number of colonies was counted from the 6th day after seeding. The colony formation rate was calculated and was shown (*P < 0.05). D. OC cells were transfected with miR-302a or control vector. Cell growth activity was determined at 12 h, 24 h and 48 h post-transfection by MTT assay. Values are means ± SD of three duplications and the relative cell growth activity is shown (*P < 0.05). E. The effect of miR-302a on apoptosis was examined by FCM analysis. OC cells were transfected with miR-302a mimics or control, and then the medium was replaced with serum-free DMEM for 48h. F. OC cells after transfected were analyzed for apoptotic rate after staining with Annexin V-FITC and PI. Data represent means ± S.D. from four independent experiments (*P < 0.05). G. The histogram showed the percentages of OC cells after miR-302a transfection in G1/S, S and G2/M phases (n=3, mean ± SD).