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. 2015 May 1;8(5):4869–4880.

Figure 3.

Figure 3

SDC1 is a directly target gene of miR-302a. A. The predicted binding sites of miR-302a on SDC1 mRNA is shown. The mutant UTR with a 4 base pair for site-directed mutagenesis in the complementary seed sequences. B. OC cells were transfected with the wild type of SDC1 reporter vector as well as miR-302a or control vector. MiR-302a suppressed the EGFP fluorescence intensity of SDC1-wt (*P < 0.05), the group transfected with SDC1-mut was not significant different to the group. C. OC cells were transfected with the mutant miR-302a or miR-302a. MiR-302a-mut could not able to significantly suppress the EGFP fluorescence intensity of SDC1-Luc (*P < 0.05). D. OC cells were transfected with miR-302a and control vector, the expression of SDC1 mRNA and protein expression level were measured by quantitative RT-PCR and Western blot. β-actin mRNA was regarded as an endogenous normalizer and the relative SDC1 mRNA expression level is shown (*P < 0.05). GAPDH protein was regarded as endogenous normalizer and the relative SDC1 protein quantity is shown (*P < 0.05).