Figure 4.

Overexpression of SDC1 could rescue the effects which miR-302a upregulated and led to cell survival in human ovarian cancer. A. We use Western blot to detect the SDC1 differential expression level after co-transfection with miR-302a and SDC1 in human ovarian cancer cells. B. OC cells were co-transfected with miR-302a and SDC1. Cell growth activity was determined at 12 h, 24 h and 48 h post-transfection by MTT assay. Values are means ± SD of three duplications and the relative cell growth activity is shown (*P < 0.05). C. OC cells were co-transfected with miR-302a and SDC1, and then seeded in 6-well plates. Colonies were counted only if they contained more than 50 cells, and the number of colonies was counted from the 6th day after seeding. The number of colonies was counted from the 6th day after seeding. The colony formation rate was calculated and was shown (*P < 0.05). D. The effect of co-transfected with miR-302a and SDC1 on apoptosis was examined by FCM analysis. OC cells after transfected were analyzed for apoptotic rate after staining with Annexin V-FITC and PI. Data represent means ± S.D. from four independent experiments (*P < 0.05). E. The histogram showed the percentages of OC cells after co-transfected with miR-302a and SDC1 in G1/S, S and G2/M phases (n=3, mean ± SD). F. Upregulation of SDC1 could block the effects which miR-302a upregulated and led to cell survival in human ovarian cancer.