Figure 3.

UFH inhibits the up-regulation of heparanase expression and activity induced by CLP. A. The mRNA level of heparanase in intestinal tissues of each group was detected by RT-PCR. The relative mRNA level was calculated using 2^-ΔΔCT method. B, C. The protein level of heparanase in intestinal tissues of each group was measured by Western blot using β-actin as internal reference. D. The activity of heparanase in intestinal tissues of each group was evaluated by HS degradation using immunohistochemistry. The results were presented as mean ± SD. **P < 0.01, ***P < 0.001. Typical results are presented.