Figure 3.

Effect of bevacizumab on endoplasmic reticulum stress pathway activation. A. The mRNA levels of IRE-1, XBP-1, CHOP and caspase-4 were examined. All RT-qPCR results were calculated and represented as the percent of vehicle control, and actin was used as a control for equal loading in the RT-qPCR experiments (#P<0.05, ##P<0.01, vs. vehicle group). B. A549 cells were incubated with bevacizumab at indicated concentrations for 24 hr. The protein of CHOP and caspase-4 were examined by Western blot. Actin was shown as a control for equal loading (#P<0.05, ##P<0.05, vs. vehicle group).