Table 1.
Primers used for PCR
| PDGFRA EXON | PRIMER | DOG1 EXON | PRIMER |
|---|---|---|---|
| EXON 1 FORWARD | gccccattgattctttcatc | EXON 1 FORWARD | cggaaaatctgaccggcg |
| EXON 1 REVERSE | aactgccactggagagcatt | EXON 1 REVERSE | tgagctcttggttgggctc |
| EXON 2 FORWARD | gggtgaatctagtggggctt | EXON 2 FORWARD | agtgagtggatgaagggagc |
| EXON 2 REVERSE | acaggagagacaggaagagaag | EXON 2 REVERSE | ggatggcctcgatctcttga |
| EXON 3 FORWARD | agtggggcaattcttctgga | EXON 3 FORWARD | ttgtggtggcctctgaagat |
| EXON 3 REVERSE | acgcaccttatgattttgcct | EXON 3 REVERSE | acatggtctttgcaggggta |
| EXON 4 FORWARD | ctgaggaatgcggtgttctg | EXON 4 FORWARD | gtgggatggttctctgacca |
| EXON 4 REVERSE | atgtgcccatcagtgacaga | EXON 4 REVERSE | gaacacgctcttctttgggg |
| EXON 5 FORWARD | tagcctcccaccttgtcaac | EXON 5 FORWARD | gtctgttccaaatgcccagg |
| EXON 5 REVERSE | ggttgacagcttccaactgg | EXON 5 REVERSE | cacagacaattagagccggc |
| EXON 6 FORWARD | atgtcagttgtccatgctgc | EXON 6 FORWARD | acacgtcactagtcaagggg |
| EXON 6 REVERSE | agtgacctgtctttccccag | EXON 6 REVERSE | caatgctgatctcaagcccc |
| EXON 7 FORWARD | tcattcagaagtcaggccgt | EXON 7 FORWARD | ttctgccaatcgagcatgtg |
| EXON 7 REVERSE | ttttcttccatgaccgggga | EXON 7 REVERSE | ttcagaggaagtcgaggcag |
| EXON 8 FORWARD | ccggagtgttttgaatgcca | EXON 8 FORWARD | tgttgccccaggatgatctt |
| EXON 8 REVERSE | acatgcagtccgactaccaa | EXON 8 REVERSE | tgctccgctccatcaactta |
| EXON 9 FORWARD | tcccaactccttgccatctt | EXON 9 FORWARD | tctttgcatcccgtgagagt |
| EXON 9 REVERSE | tctgccttgggaccttcatt | EXON 9 REVERSE | gcttcatctaatgctggccc |
| EXON 10 FORWARD | cagacacagccacactacct | EXON 10 FORWARD | ctgcttctgggatgagggaa |
| EXON 10 REVERSE | gtgtgcaagggaaaagggag | EXON 10 REVERSE | atgtcctttcctcctgacgg |
| EXON 11 FORWARD | tggtgctgttggtgattgtg | EXON 11 FORWARD | cccgtcccattgtgtgtttt |
| EXON 11 REVERSE | gtgtgcaagggaaaagggag | EXON 11 REVERSE | aagacagacagaagcagcct |
| EXON 12 FORWARD | ctcctttctcccgtctgtgt | EXON 12 FORWARD | agaatcgcttgaacccagga |
| EXON 12 REVERSE | ccaacatacaggcagcaaga | EXON 12 REVERSE | tgtccatttcctctccaccc |
| EXON 13 FORWARD | gcactgaggccaagtagcta | EXON 13 FORWARD | tcaccctcaagcagcagtaa |
| EXON 13 REVERSE | atgtgtggggatggagagtg | EXON 13 REVERSE | agggtgctggaaggaaagaa |
| EXON 14 FORWARD | actctccatccccacacatg | EXON 14 FORWARD | tgttctcagggccagttcat |
| EXON 14 REVERSE | aagactggacagggtggttt | EXON 14 REVERSE | taccatggcagagttgagca |
| EXON 15 FORWARD | ccagttagctcccatgccta | EXON 15 FORWARD | tttaaaacgcccagaacccg |
| EXON 15 REVERSE | gaactggttgtgcagacctg | EXON 15 REVERSE | tggcatgcatgttaacgagg |
| EXON 16 FORWARD | cctcctccctgattcaagca | EXON 16 FORWARD | gcttcttggaaccctatgcg |
| EXON 16 REVERSE | gtccacactccactcactga | EXON 16 REVERSE | aagccaatcacttccttgcg |
| EXON 17 FORWARD | ctctccttcatcccctacgc | EXON 17 FORWARD | ccatctgtctctggtgggtt |
| EXON 17 REVERSE | caatcagggagacagagggg | EXON 17 REVERSE | acccattgagaaccagggag |
| EXON 18 FORWARD | tgtggccgtgatagctgtaa | EXON 18 FORWARD | ctccctggttctcaatgggt |
| EXON 18 REVERSE | cctggggcttgaaagaacac | EXON 18 REVERSE | tggggaaaggctgaagatgt |
| EXON 19,20 FORWARD | aaaaccagtgcttcaaggct | EXON 19 FORWARD | ttgccttaatcccctcctgg |
| EXON19,20 REVERSE | gctcctctagaactgcggaa | EXON 19 REVERSE | tgcacccgtccctcattaat |
| EXON 21 FORWARD | tggccccttcttcatgttct | EXON 20 FORWARD | acaccccagcatgaaaacac |
| EXON 21 REVERSE | tctgcaacttgtccctgagt | EXON 20 REVERSE | cctccttctctccctgcttc |
| EXON 22 FORWARD | gctttcgtttgtctctgggg | EXON 21 FORWARD | gcaacactgtgaaaccccat |
| EXON 22 REVERSE | gacatcaggtttcaagaggga | EXON 21 REVERSE | tgcatctctcttctccctgc |
| EXON 22 FORWARD | ggtccatcccaagagctcat | ||
| EXON 22 REVERSE | caaacccagcacgcactc | ||
| EXON 23 FORWARD | cctcaacagcaaagccatgt | ||
| EXON 23 REVERSE | ccaaggtccctgatgtcact | ||
| EXON 24 FORWARD | accctgcatccattcactca | ||
| EXON 24 REVERSE | cacaacgcccggctatttaa | ||
| EXON 25 FORWARD | agtaggctctgcactcttgg | ||
| EXON 25 REVERSE | agcaagttctccaagcctct | ||
| EXON 26 FORWARD | aaagtcggccaacatttccc | ||
| EXON 26 REVERSE | tgccgtattaccagccatca |