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Expression of miR-144 in tissues and peripheral blood of malignant SPN patients. Total RNA was extracted using Trizol reagent and the integrity of RNA was tested using gel electrophoresis. The purity of RNA was determined by A260/A280 using ultraviolet spectrophotometry. Then, cDNA was obtained by reverse transcription. QRT-PCR was performed using SYBR PrimeScript RT-PCR kit. *, P < 0.05 compared with negative control group.
