Figure 2.

Serum-starved cells were given IGF-I (10 ng/ml) with or without preincubation with 50 mM LY294002 (LY) for 10 min. The proliferation capacity was measured by absorbance value using a microplate reader. A. Cells were harvested 24 h after stimulation or inhibition. B. Cells were harvested 48 h after stimulation or inhibition. C. Cells were harvested 72 h after stimulation or inhibition. Results are expressed as the means ± SD; ***P < 0.001, **P < 0.01. The representative results from three independent experiments are shown.