Figure 3.

Migration and Invasion of MCF-7 cells in response to IGF-I and LY294002 A. control group. B. IGF-1 group. C. IGF-1+LY group. D. LY group. Serum-starved cells were given IGF-I (10 ng/ml) with or without pre-incubation with 50 mM LY294002 (LY) for 10 min. The migration and invasion assay was measured by scratch-wound assay. Subconfluent cultures of MCF-7 cells were wounded with a sterile pipette tip and repopulation of the wounded area by migrating cells observed 24 hour later. The extent of repopulation in three repeat experiments was quantitated using Image J software.