Table I.

HSPA4 mRNA expression levels in patient derived plasma cells CD138-positive cells were isolated from bone marrow sample of the myeloma patient as described before and cells were either kept in suspension (SUS) or adhered to FN for 2 h in serum-free medium. After 2 h, non-adherent cells were aspirated and adherent cells were kept in 15% αMEM. The total RNA was extracted using RNA easy kit and RT-PCR analysis was conducted for HSPA4 RNA.

Disease status	mRNA copies of HSPA4		HSPA4	% Myeloma cells
	SUS	FN
ND	2380	990	D	90
ND	2121	740	D	60
ND	411	504	NC	45–50
ND	649	668	NC	40
ND	522	487	NC	40
ND	276	287	NC	99
ND	414	406	NC	50
ND	2177	2476	I	65–70
R	395	504	I	50
R	5852	10 379	I	85
R	8602	2443	D	30–40
R	958	1406	I	90
R	4434	4818	I	65
R	1978	11 925	I	33
R	1453	2092	I	25
R	1913	3204	I	50

ND, newly diagnosed; R, relapsed; D, decreased; NC, no change; I, increased.