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Western blot analysis of human kidney lysates. Representative experiments with human kidney lysates under nonreducing conditions (lane a) and under reducing conditions (lane b), along with molecular mass markers (lane c). hOCT2 proteins were detected with an anti-hOCT2 antibody, which recognizes an epitope of the extracellular loop. Each experiment was repeated ≥3 times in independent human kidney preparations, resulting in qualitatively similar results.
