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. 2014 Feb 6;9(3):566–571. doi: 10.1002/cmdc.201300428

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© 2014 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

© 2014 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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n-Octyl ester 5 increases H3K9me3 levels in HeLa cells via KDM4A inhibition. Indirect immunofluorescence assays with anti-Flag (green) and anti-H3K9me3 (red) antibodies and with DAPI staining (blue) in HeLa cells overexpressing Flag-tagged KDM4A. DMSO treatment has no effect on KDM4A demethylase activity, while IOX1 (1) (300 μm) and n-octyl ester 5 (10 μm) treatment resulted in increased H3K9Me3 substrate levels (white arrows indicate transfected cells). The H188A catalytically inactive KDM4A variant does not affect H3K9Me3 levels.