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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Nat Rev Genet. 2015 Apr 9;16(5):299–311. doi: 10.1038/nrg3899

Figure 3. Screening strategies in either arrayed or pooled formats.

Figure 3

Genetic screens follow two general formats that differ in the way in which the targeting reagents are constructed and how cell targeting and readout is carried out. a | In arrayed screens, reagents are separately synthesized and targeting constructs are arranged in multiwell plates. Cell targeting is also conducted in multiwell plates using either transfection or viral transduction. Screen readout is based on cell population measurements in individual wells. b | In pooled screens, reagents are usually synthesized and constructed as a pool. Viral transduction limits transgene copy number (ideally, one perturbation per cell), and viral integration enables readout through PCR and next-generation sequencing. Readout is based on the comparison of the abundance of the different genomically integrated transgene reagents between samples. MOI, multiplicity of infection; sgRNA, single guide RNA; shRNA, short hairpin RNA; siRNA, small interfering RNA.