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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Nat Rev Genet. 2015 Apr 9;16(5):299–311. doi: 10.1038/nrg3899

Table 1.

Experimental parameters of recent Cas9-mediated genetic screens

Cas9 delivery Cas9 protein sgRNA
library size		 Number of
targeted genes		 Coverage (sgRNAs
per gene)		 Cell
lines		 Species Positive or
negative
selection		 Refs
Clonal isolation of
stably integrated cells		 Cas9 nuclease 73,151 7,114 10 and tiling sgRNAs
for ribosomal genes		 KBM7;
HL60		 Human Both 45
Delivery with the
sgRNA library		 Cas9 nuclease 64,751 18,080 3 or 4 on average A375;
HUES62		 Human Both 44
Clonal isolation of
stably integrated cells		 Cas9 nuclease 87,897 19,150 4 on average mESC Mouse Both 46
Clonal isolation of
stably integrated cells		 Cas9 nuclease 873 291 3 HeLa Human Positive 47
Polyclonal selected cell
Population		 dCas9 repression
complex		 206,421 15,977 10 per TSS K562 Human Both 48
Polyclonal selected cell
Population		 dCas9 activation
complex		 198,810 15,977 10 per TSS K562 Human Both 48
Polyclonal selected cell
Population		 dCas9 activation
complex		 70,290 23,430 3 per TSS A375 Human Both 49

dCas9, catalytically inactive Cas9; mESC, mouse embryonic stem cell; sgRNA, single guide RNA; TSS, transcription start site.