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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Acta Biomater. 2015 Apr 22;22:103–110. doi: 10.1016/j.actbio.2015.04.013

Table 1.

Primers used for real-time RT-PCR

Gene Primer sequence Efficiency
Tbp F: 5′-GCGCAAGGGTTTCTGGTTTGCC-3′
R: 5′-AGGGATTCCGGGAGTCATGGC-3′		 1.93
Nkx6.1 F: 5′-GCTCTGTCTCCGAGTCCTG-3′
R: 5′-TCGTTGGGGATGACAGAGA-3′		 1.98
Pdx1 F: 5′-TACTGGATTGGCGTTGTTTGTGGC-3′
R: 5′-AGGGAGCCTTCCAATGTGTATGGT-3′		 1.91