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. Author manuscript; available in PMC: 2015 Jul 15.
Published in final edited form as: J Cell Biochem. 2011 Jan;112(1):225–232. doi: 10.1002/jcb.22915

Figure 2. Reciprocal IP/Western analysis for the association of phosphorylated RNAPII in p27SJ-expressing cells.

Figure 2

A. Results from IP/western-blot analysis of extracts from the cells expressing p27SJ. Lysates were immunoprecipitated with anti-p-CTD RNAPII and analyzed by Western blot assay using anti-p27SJ antibody. The negative control was nonimmune rabbit serum. B. Results from IP/western-blot analysis of extracts from the cells expressing p27SJ. Lysates were immunoprecipitated with anti-p27SJ antibody and analyzed by Western blot assay using anti-p-CTD RNAPII. The negative control was nonimmune rabbit serum.