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. Author manuscript; available in PMC: 2015 Jul 15.
Published in final edited form as: J Cell Biochem. 2011 Jan;112(1):225–232. doi: 10.1002/jcb.22915

Figure 4. Analysis of the effect of p27SJ expression on LTR transcription elongation.

Figure 4

A. Schematic representation of the HIV-LTR-Luciferase reporter plasmid containing the luciferase gene under the control of LTR that was used in the transcription elongation studies. The sites of the primers used in the RT-PCR elongation assays are specified as are the positions of the three amplicons. B. Effect of p27SJ on LTR transcription elongation was analyzed in RT-PCR assays for the three amplicons. The negative control was performed without reverse transcriptase. The image of the ethidium bromide-stained gel was inverted using Adobe Photoshop for clarity of presentation. C. The integrity and loading of each of the RNA samples from cells with or without p27SJ was examined by agarose gel electrophoresis and ethidium bromide staining. The image of the gel was inverted using Adobe Photoshop for clarity of presentation.