Skip to main content
. Author manuscript; available in PMC: 2015 Jul 15.
Published in final edited form as: J Cell Biochem. 2009 Dec 1;108(5):1117–1124. doi: 10.1002/jcb.22343

Figure 5. Induction of endogenous BAG3 by C-terminal BAG in T98G cells.

Figure 5

(A) Time course of C-terminus 1x overexpression in T98G cells. After transfection, cells were harvested at indicated time points. Forty micrograms of lysate was subjected to Western blot and probed with rabbit polyclonal antibody raised against the full-length BAG3 protein. (B) Time course of C-terminus 2X overexpression in T98G cells. The procedure was performed as in Panel A. (C) qRT-PCR analysis of RNA in the same samples as in Panel B. The BAG3 transcript was measured and β-Actin mRNA was used as the normalization control