Skip to main content
. Author manuscript; available in PMC: 2015 Jul 15.
Published in final edited form as: J Cell Biochem. 2009 Dec 1;108(5):1117–1124. doi: 10.1002/jcb.22343

Figure 6. Subcellular localization of BAG3.

Figure 6

(A) Untransfected cells and cells (U-87 MG and T98G) transfected with BAG3 FL were harvested after 24 h and nuclear-cytoplasm fractionated as described in Materials and Methods. Five and 15 μg of nuclear and cytoplasmic fractions, respectively, were evaluated by Western blot and probed with a rabbit polyclonal antibody against BAG3. The position of BAG3 is shown by an arrow. An asterisk points to a non-specific band. The purity of fractionation was checked by hybridization with anti-Lamin A/C and anti-α-tubulin antibodies. (B) U-87 MG cells were transfected as in A, hybridized with polyclonal BAG3 antibody and FITC-labeled secondary anti-rabbit, and microscopically analyzed.