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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Biomaterials. 2015 Jun 20;64:88–97. doi: 10.1016/j.biomaterials.2015.06.038

Fig. 3.

Fig. 3

Human SDSCs expanded on either dECM or Plastic for one passage were followed by a 28-day chondrogenic induction in a pellet system with or without preconditioning of sb203580. (A) Pellets were photographed before histology; Alcian blue (Ab) was used to stain sulfated GAGs and immunohistochemical staining (IHC) was for Col II. (B) Biochemical analysis was used for DNA and GAG amounts in chondrogenic pellets. Cell proliferation and viability was evaluated using DNA ratio (DNA amount at days 28 and 14 adjusted by that at day 0). Chondrogenic index was evaluated using ratio of GAG to DNA. Data are shown as average ± SD for n = 4. * p < 0.05 suggests a statistically significant difference.