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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Biomaterials. 2015 Jun 20;64:88–97. doi: 10.1016/j.biomaterials.2015.06.038

Fig. 6.

Fig. 6

35-day-chondrogenic induction of dECM-expanded hSDSCs preconditioned with sb203580 in the presence of 1 ng/mL IL-1β. Human SDSCs were expanded on either Plastic or dECM for one passage with or without preconditioning using sb203580 followed by a 35-day pellet culture in a serum-free chondrogenic medium supplemented with continuous treatment of IL-1β at 1 ng/mL. (A) Alcian blue (Ab) was used to stain sulfated GAGs. Immunohistochemistry staining (IHC) was used to detect collagen II (Col II). (B) Biochemical analysis was used for DNA and GAG amounts in the 35-day chondrogenically-induced pellets. Chondrogenic index was evaluated using a ratio of GAG to DNA. (C) Real-time PCR was used to evaluate chondrogenic marker gene expression (ACAN and COL2A1) in day 35 pellets. (D) Real-time PCR was used to evaluate inflammatory marker gene expression (PTGS2, MMP13, and VEGFA) in day 35 pellets. Data are shown as average ± SD for n = 4. * p < 0.05 compared with the corresponding Plastic group. # p < 0.05 compared with the corresponding group without sb203580 treatment.