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. Author manuscript; available in PMC: 2015 Dec 11.
Published in final edited form as: Nature. 2015 Apr 20;522(7555):221–225. doi: 10.1038/nature14308

Extended Data Figure 5. TEM analyses of hECCs and control embryo staining (supporting Fig. 3).

Extended Data Figure 5

a) TEM analysis of hECCs (NCCIT) with heavy metal staining, arrow indicates VLPs. Boxed region is shown with higher magnification in an inset. Scale bar = 500 nm. Shown is a representative example of two independent experiments.

b) TEM immuno-gold labeling of hECC (NCCIT) with Gag/Capsid antibodies. Shown is a representative example from 2 independent experiments.

c) Secondary only control for Immuno-gold labeling of human blastocysts. Shown is a representative example from 8 fields of view.

d) Model figure summarizing HERV-K transcriptional regulation in human embryos and in vitro cultured pluripotent cells. Dashed lines indicate inference of OCT4, DNA methylation and HERV-K level changes at implantation from those observed between naïve and primed hESCs, in the absence of data from actual postimplantation human embryos.