Thirteen adult hu-BLT mice were randomly divided into early treatment (Rx-6h, Rx-24h, Rx-48h, n=3 each) and control (n=4) groups. All animals were inoculated intraperitoneally (IP) with a mixture of two transmitted/founder HIV-1 viruses (2.5 × 103 TCID50 each). Daily ART was initiated at 6, 24 or 48 hours post-infection for each respective treatment groups. Three weeks after ART was stopped, CD8+ T-cells from all animals were depleted. The levels of CD8+ T-cells, CD4+ T-cells, PVL and vDNA in tissue were monitored as indicated.