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. Author manuscript; available in PMC: 2015 Jul 15.
Published in final edited form as: Neuroscience. 2014 Apr 26;271:108–118. doi: 10.1016/j.neuroscience.2014.04.033

Figure 1.

Figure 1

Schematic of a typical ELL slice as it would appear in a recording chamber. The tractus stratum fibrosum (StF) is readily apparent in vitro and presents a landmark located just above the pyramidal cell layer (PCL). The slice is optimally placed for the artificial cerebrospinal fluid (ACSF) to flow over the molecular layer of the ELL (Dorsal Molecular Layer, DML and Ventral Molecular Layer, VML) as shown by the arrow. Accordingly, picospritzer pipettes were placed in the DML to allow for both ket and 5-HT diffusion across DML, VML, StF, PCL. GCL: granule cell layer.