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. 2015 Jul 15;10(7):e0133385. doi: 10.1371/journal.pone.0133385

Fig 1. 4T1- and LLC-conditioned macrophages exhibit increased pro-inflammatory cytokine production in response to LPS.

Fig 1

(A) C57BL/6 or (B) BALB/c BMDMs were cultured in DMEM alone or in the presence of culture medium conditioned by 4T1 mammary carcinoma, E0771 mammary carcinoma, ID8 ovarian carcinoma, or LLC cells for 24 h. Medium conditioned by HC11 normal mouse epithelial cells was used as a negative control. BMDMs were then washed with PBS and stimulated with 100 ng/ml LPS for 4 h, then culture supernatants were collected and protein levels of cytokines TNFα, IL-6, CCL2, and IL-10 were determined by ELISA. Results are shown as the mean (± SEM) of at least 5 independent experiments. Statistical analyses were done by ANOVA, followed by Tukey’s multiple comparison tests; * p < 0.05, ** p < 0.01, *** p < 0.001.