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. 2015 Jul 15;10(7):e0133385. doi: 10.1371/journal.pone.0133385

Fig 3. Increased sensitivity of 4T1-conditioned macrophages to LPS is transient.

Fig 3

BMDMs were treated with 4T1- or HC11-conditioned medium (CM) for the indicated times. Following treatment, BMDMs were then washed with PBS and stimulated with 100 ng/ml LPS for 4 h. Culture supernatants were collected and protein levels of cytokines CCL2, TNFα, and IL-6 were determined by ELISA. Results are the mean (± SEM) of 3 independent experiments. Statistical analyses were done by ANOVA, followed by Tukey’s multiple comparison tests; * p < 0.05.