Table 1. Effect of 100 μM AS and 5 μM CX treatment on callus induction and transformation efficiency in L. japonicas accession MG-20.

Experimet		segments	Hygromycin-resistant calli a	Callus induction rate (%) b	Hygromycin-resistant plants	PCR-positive plants	Transformation efficiency (%) c
1	control	100	28	28.0	6	0	0.0
	AS	75	14	18.7	3	0	0.0
	CX	100	45	45.0	3	0	0.0
2	control	225	61	27.1	10	1	0.4
	AS	225	31	13.8	12	2	0.9
	CX	225	118	52.4	12	3	1.3
Total	control	325	89	27.4	16	1	0.3
	AS	300	45	15.0	15	2	0.7
	CX	325	163	50.2	15	3	0.9

^a Number of calli resistant to 50mg/L hygromycin

^b (Number of hygromycin resistant calli / Number of segments) X 100

^c (Number of PCR-positive plants / Number of segments) X 100