Fig 7. Effect of BAR502 on cholestasis induced by 17αE₂.

C57BL6 mice were treated for 8 days with 17αE₂ or with the combination of 17αE₂ plus BAR502 as described in materials and methods. (A) Body weight. (B) Scratching tests were performed at day 8. Mice were subjected to intradermal injection of DCA and scratching events were measured during 60 minutes of observation. (C) Serum levels of bilirubin. (D) Serum levels of Alkaline phosphatase. (E) Serum levels of AST. Results are the mean ± SE of 4–8 mice per group. *p<0.05 versus control group (CTRL). #p<0.05 versus 17αE₂ treated mice. (F-H) Representative histological pictures of H&E-stained livers.