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. 2015 Jul 15;10(7):e0132480. doi: 10.1371/journal.pone.0132480

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© 2015 Song et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 1 — Ferritin-expressing lentiviral vectors were constructed in two steps. First, the FTH1 sequence was cloned into the pLVTHM vector between the MluI and ClaI sites, downstream of the H1p promoter, generating the pLVTHM-H1p-FTH1 plasmid vector. Second, one of three neural cell-specific promoter sequences (SYN1p, GFAPp, or MBPp) was cloned into the pLVTHM-H1p-hFTH1 vector between the EcoRI and MluI sites, replacing the H1p sequence, to generate three NDIFE vectors: pLVTHM-SYN1p-FTH1, pLVTHM-GFAPp-FTH1, and pLVTHM-MBPp-FTH1. FTH1: ferritin heavy chain, SYN1: synapsin I, GFAP: glial fibrillary acidic protein, MBP: myelin basic protein.