Figure 3.
DU145 and HFL-1 (normal human fibroblasts) cells were treated with increasing concentrations of 28 or DMSO (Vehicle) for 48 h. Cells were harvested and total protein was resolved by 10% SDS-PAGE, western blotted and hybridized to anti-PARP (Cell Signaling # 542) anti body. The blot was then washed and treated with anti-rabbit secondary labeled with infrared dye (700) and scanned using the Odyssey scanner (LiCor).