Table 1. Oligonucleotide primers used for amplification of ORF3 mutants.

The lowercase letters in the universal sense primer represent the Kozak sequence and those in the antisense primers signify mutagenic nucleotides altering single P to A. N/A, not applicable.

Primer	Sequence	Restriction site	Mutagenic nucleotides
universal sense primer		5ʹ-CCGGAATTCgccaccATGgcaCCACCATGCGCT-3ʹ	EcoR I	N/A
Anti-sense primers	Wild type	5ʹ-CCGCTCGAGACGGCGAAGCCCCAGC-3ʹ	XhoI	N/A
	P99A	5ʹ-CCGCTCGAGACGGCGAAGCCCCAGCTGGGGCAGATCGACGACGGGCGGGAGCGGagcGG-3ʹ	XhoI	cgg → agc
	P100A	5ʹ-CCGCTCGAGACGGCGAAGCCCCAGCTGGGGCAGATCGACGACGGGCGGGAGagcCG-3ʹ	XhoI	cgg → agc
	P102A	5ʹ-CCGCTCGAGACGGCGAAGCCCCAGCTGGGGCAGATCGACGACGGGagcGA-3ʹ	XhoI	cgg → agc
	P103A	5ʹ-CCGCTCGAGGGCGAAGCCCCAGCTGGGGCAGATCGACGACagcCG-3ʹ	XhoI	ggg → agc