Fig 5. Knockdown of PABPC1 inhibits activation of the PSA promoter by NAR.

(A) Schematic diagram of GFP-NAR. (B) C4-2 cells were transfected with control siRNA (siC) or siPABPC1 (pool of 3 oligos) (40 pmol/mL) for 48 hours followed by transfection with PSA6.1Luc, pRL-CMV, and either GFP (control) or GFP-NAR in the presence of 5% charcoal stripped FBS RPMI for an additional 48 hours followed by luciferase assay. Experiments were repeated two times. Significance was determined by Student’s t-test (**p<0.01, ***p<0.001).