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. 2015 Jul 15;10(7):e0132941. doi: 10.1371/journal.pone.0132941

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© 2015 Zhang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 1 — (A) In cumulus-oocyte complexes (COCs), the peripheral layer of the cumulus expanded to more than 5 layers in the control group, whereas it became progressively thinner in the NAM- and Sirtinol-treated COCs. Most oocytes in the control group extruded the first polar body (indicated by white arrow). Upon treatment with NAM and Sirtinol, a large proportion of oocytes failed to extrude polar bodies. Bar = 100 μm. (B) Rate of polar body extrusion in the control, NAM-, and Sirtinol-treated groups. Rate of polar body extrusion in treatment groups was significantly reduced comparing to control group. *, significant, p < 0.05.