Table 1.
Differentially regulated genes in Men1-KO versus WT adipocytes derived from mESCs.
| Affymetrix probe set ID | Ref seq. transcript ID | Gene symbol | Gene title | Microarray fold change | QPCR fold change |
|---|---|---|---|---|---|
| 1451634_at | NR_002853 | Airn | Antisense Igf2r RNA | −9.24 | −18.5 |
| 1457030_at | NR_028265 | Mirg | miRNA containing gene | −8.36 | −6.54 |
| 1438588_at | NM_009538 | Plagl1 | Pleiomorphic adenoma gene-like 1 | −7.04 | −7.46 |
| 1427580_a_at | NR_028261 | Rian | RNA imprinted and accumulated in nucleus | −6.96 | −10.7 |
| 1436713_s_at | NR_003633 | Meg3 | Maternally expressed gene 3 | −6.72 | −7.35 |
| 1417836_at | NM_024198 | Gpx7 | Glutathione peroxidase 7 | −5.91 | −6.49 |
| 1421461_at | NM_001122949 | Mpl | Myeloproliferative leukemia virus oncogene | −5.68 | −1.74 |
| 1418061_at | NM_013589 | Ltbp2 | Latent transforming growth factor beta binding protein 2 | −5.51 | −2.28 |
| 1428896_at | NM_026840 | Pdgfrl | Platelet-derived growth factor receptor-like | −4.52 | −2.9 |
| 1419411_at | NM_009312 | Tac2 | Tachykinin 2 | −4.34 | −3.29 |
| 1423506_a_at | NM_010923 | Nnat | Neuronatin | −3.92 | −2.86 |
| 1460412_at | NM_024237 | Fbln7 | Fibulin 7 | −3.91 | −7.51 |
| 1448788_at | NM_010818 | Cd200 | CD200 antigen | −3.76 | −4.5 |
| 1421074_at | NM_007825 | Cyp7b1 | Cytochrome P450, family 7, subfamily b, and polypeptide 1 | −3.73 | −3.09 |
| 1419197_x_at | NM_032541 | Hamp | Hepcidin antimicrobial peptide | −3.73 | −2.84 |
| 1417979_at | NM_022322 | Tnmd | Tenomodulin | −3.63 | −3.36 |
| 1425040_at | NM_028593 | Cybrd1 | Cytochrome b reductase 1 | −3.57 | −4.69 |
| 1419292_at | NM_001042615 | Htra3 | HtrA serine peptidase 3 | −3.49 | −5.69 |
| 1435476_a_at | NM_001077189 | Fcgr2b | Fc receptor, IgG, low affinity IIb | −3.27 | −8.81 |
| 1416347_at | NM_001168488 | Men1 | Multiple endocrine neoplasia 1 | −3.18 | −714.1 |
| 1450285_at | NM_011667 | Ube1y1 | Ubiquitin-activating enzyme E1, Chr Y 1 | 4.34 | 9.38 |
| 1435064_a_at | NM_020626 | Tmem27 | Transmembrane protein 27 | 4.39 | 9.44 |
| 1448783_at | NM_021291 | Slc7a9 | Solute carrier family 7 (cationic amino acid transporter, y+ system), member 9 | 4.43 | 17.26 |
| 1456601_x_at | NM_007503 | Fxyd2 | FXYD domain-containing ion transport regulator 2 | 4.51 | 19.42 |
| 1424279_at | NM_001111048 | Fga | Fibrinogen alpha chain | 4.74 | 7.31 |
| 1418916_a_at | NM_029269 | Spp2 | Secreted phosphoprotein 2 | 4.86 | 19.56 |
| 1449907_at | NM_001163028 | Bcmo1 | Beta-carotene 15,15′-monooxygenase | 4.92 | 2.84 |
| 1422289_a_at | NM_029636 | Ctsq | Cathepsin Q | 4.94 | 6.32 |
| 1418766_s_at | NM_001161355 | Timd2 | T-cell immunoglobulin and mucin domain-containing 2 | 5.2 | 13.92 |
| 1443824_s_at | NM_053070 | Car7 | Carbonic anhydrase 7 | 5.39 | 5.89 |
| 1417761_at | NM_007468 | Apoa4 | Apolipoprotein A-IV | 5.59 | 7.67 |
| 1418724_at | NM_007686 | Cfi | Complement component factor i | 5.86 | 9.12 |
| 1440218_at | NM_001033364 | Cdhr2 | Cadherin-related family member 2 | 6.02 | −1.07 |
| 1452165_at | NM_025532 | Prl2b1 | Prolactin family 2, subfamily b, member 1 | 6.06 | 19.69 |
| 1451513_x_at | NM_009243 | Serpina1a | Serine (or cysteine) peptidase inhibitor, clade A, member 1A | 6.28 | 12.46 |
| 1418282_x_at | NM_009244 | Serpina1b | Serine (or cysteine) peptidase inhibitor, clade A, member 1B | 6.34 | 6.02 |
| 1424673_at | NM_053165 | Clec2h | C-type lectin domain family 2, member h | 7.19 | 7.11 |
| 1429826_at | NM_029355 | Prl7b1 | Prolactin family 7, subfamily b, member 1 | 7.58 | 29.44 |
| 1448572_at | NM_011165 | Prl4a1 | Prolactin family 4, subfamily a, member 1 | 15.49 | 29.65 |
| 1436717_x_at | NM_008221.4 | Hbb-y | Hemoglobin Y, beta-like embryonic chain | 19.31 | 2.8 |
Validation of top 20 downregulated or upregulated genes (p < 0.001) identified in the gene expression microarray analysis. QPCR fold change was normalized to Gapdh. Bold, genes regulated by differential DNA methylation. Italic, genes from the prolactin gene family.