Table 1. RNAi-screening for essential TFIIS genes.

Targeted gene		TFIIS1a	TFIIS1c	TFIIS2a	TFIIS2b	TFIIS2a and TFIIS2b	TFIIS3	TFIIS4	ND7	ICL7	none
Progeny	% wild type	94	91	94	86	89	90	15	97	97	87
	% sick	1	0	1	2	3	2	10	0	0	2
	% death	5	9	5	12	8	8	75	3	3	11
Total cells		192	192	192	192	192	192	493	282	210	222
Number of experiments		3	3	3	3	3	3	13	6	7	4

Survival test of post-autogamous cells submitted to RNAi against all TFIIS genes and control non-essential genes—ICL7 and ND7. The last column “none” corresponds to the control grown in standard non-feeding K. pneumoniae medium. For each condition, the number of replicate experiments is indicated in the last line. In one replicate experiment, wild-type survivors were systematically tested for MAC regeneration (as previously described [52]) and all turned out to be true postautogamous cells.