Table 2.
Flow cytometry analysis.
| Blood % | Liver % | Abdominal fat % | Subcutaneous fat % | |||||
|---|---|---|---|---|---|---|---|---|
| (median (range)) | (median (range)) | (median (range)) | (median (range)) | |||||
| ND | HFD | ND | HFD | ND | HFD | ND | HFD | |
| CD45+ | 81 (42.3–78.4) | 86.1 (69.2–96.3) | 66.8 (54.2–78.4) | 60 (48.6–71) | 35.6 (26.8–73.6) | 30.3 (19.8–78.6) | 73.9 (32.5–95.61) | 74.2 (45.8–46.62) |
| CD45+CD3+ | 14 (8.4–11.3) | 9.5 (4.9–17.1) | 13.6 (7.9–32.4) | 18.8 (11.7–46.9) | 14.8 (6–61.5) | 11.7 (5.4–27.6) | 33.1 (13.4–51.6) | 24.7 (11.9–28.3) |
| CD45+CD3+CD4+ | 42.6 (14.6–39.7) | 39.7 (30.1–52.5) | 33 (27.4–35.6) | 29.1 (9.2–41.4) | 45.9 (40.2–56.2) | 29.4 (14.7–39.8)∗ | 37.4 (28.1–41.6) | 41.5 (31.1–46.6) |
| CD45+CD3+CD4+FOXP3+ | 9.6 (6.3–10.9) | 10 (7.6–15.3) | 5.4 (3.3–32.6) | 8.5 (4.6–45) | 22.8 (13.5–45.6) | 16 (10.7–20.1)∗ | 8.2 (0.8–13.2) | 11.7 (7.6–18.2) |
| Tregs (CD4+CD25+FOXP3+) | 3.5 (1.7–5.5) | 3 (2–4.4) | 0.14 (0.06–0.6) | 0.2 (0.6–3.3) | 1.4 (0.6–6.7) | 3 (0.6–4.3) | 0.6 (0.3–1.3) | 1.3 (0.8–3.5)∗ |
| CD4+RORγt++ | 1.7 (0.5–6.7) | 2.2 (0.7–5) | 2.4 (0.9–5.1) | 9.7 (1.5–24.6)∗ | 2.3 (0.7–5.8) | 8.7 (2.7–15.4)∗ | 3.6 (1–18.5) | 4.5 (2–10.2) |
Flow cytometric analysis of blood, liver, abdominal fat, and subcutaneous fat in mice fed high fat diet (HFD) and normal diet (ND). Cells are represented as % [(median (range)] of the gated population: CD45+CD3+ were gated on CD45+, CD45+CD3+CD4+ were gated on CD45+CD3+, CD45+CD3+CD4+FOXP3+ were gated on the CD45+CD3+CD4+, Tregs (CD45+CD3+CD4+CD25+FOXP3+) were gated on the CD45+CD3+CD4+, and CD45+CD3+CD4+RORγt++ were gated on the CD45+CD3+CD4+. ∗Statistically significant difference between mice fed HFD and ND; p < 0.05.