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. 2015 Jul 15;10(7):e0132702. doi: 10.1371/journal.pone.0132702

Fig 1. Flow cytometry analysis of γH2AX-stained DNA double-strand breaks.

Fig 1

Mean γH2AX intensity was assessed in PBMCs immediately, 1 h and 20 h after indicated exposure conditions. (A) Representative overlay histogram of γH2AX-intensity 1 h after indicated exposure (black line) and of corresponding control (gray line). (B) Difference of mean fluorescence intensity (MFI) of γH2AX and IgG-isotype control staining from 16 independent experiments at three different time points after exposure as mean ± SEM (***: P ≤ 0.001; ns: P > 0.05).