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. 2015 Jul 15;10(7):e0133266. doi: 10.1371/journal.pone.0133266

Fig 4. ETA mediates endothelial hyper-permeability.

Fig 4

(A-B) Translocation of FITC-albumin across EAhy monolayers plated in duplicate in Transwell inserts was monitored over time following treatment with vehicle alone, ET-1 (1 μM, A) thrombin (2 U/ml, B). Data are mean ± S.E.M. of 2–3 individual experiments. (C-D) Cells were stimulated with thrombin or ET-1 for the indicated times followed by fixation and immunostaining with phalloidin (F-actin, red, C) or VE-cadherin antibody (red, D). Images are from a single experiment representative of 3 similar experiments.