Fig 6. SCLS-associated mediator CXCL10 induce Ednra expression in endothelial cells.

(A-B) EAhy cells were left untreated (0 h) or stimulated with CXCL10 (40 ng/ml) for the indicated times followed analysis of Ednra expression by qPCR. Data are average of duplicate measurements from a single experiment (A) or mean ± S.E.M of four individual experiments (B); ***p = 0.0004; t test. (C) Edrna expression in BOEC from SCLS patients or controls (n = 4/group) left untreated or stimulated with CXCL10 (40 ng/ml) for 4 hr was determined by qPCR. Data are mean ± S.E.M.; ***p = 0.005, two-way-ANOVA. (D) BOEC from a subject with SCLS or healthy donor were pretreated with CXCL10 (40 ng/ml) for four hours prior to stimulation with the indicated concentrations of ET-1 or ionomycin and measurement of intracellular Ca²⁺ concentrations by fluorimetry. Bar graph is mean ± S.E.M. of quadruplicate values from a single experiment representative of 3 similar experiments.