Skip to main content
. 2015 Jul 15;10(7):e0132845. doi: 10.1371/journal.pone.0132845

Fig 1. Validation of exosome isolation.

Fig 1

(A) Acetylcholinesterase activity assays were used to detect exosomes in isolates from untreated PANC-1 cells (curcumin-negative exosomes) or PANC-1 cells treated with 50 μM of curcumin for 24 hours (curcumin-positive exosomes) compared to assay diluent, 1X PBS (control). (B) Nicomp dynamic light scattering (DLS) analysis was used to measure size distribution of particles in exosome isolates. (C) NanoSight nanoparticle tracking analysis (NTA) was used to confirm size distribution of particles in exosome isolates. (D) Particle concentration (particles/mL) was measured using NanoSight NTA. No significant differences were observed in acetylcholinesterase activity, size distribution, or particle concentration between curcumin-negative exosomes and curcumin-positive exosomes. Data are represented as mean ± SEM of three independent experiments, *p<0.05, **p<0.01, exosome fraction versus control.