| pTW438-CeOCT-1-MYC–F |
Gap repair into the expression plasmid pTW438 |
5'-CTGCACAATATTTCAAGCTATACCAAGCATACAATAAGCTTATGT CTGCAACTAAACCTCCA |
| pTW438-CeOCT-1-MYC–R |
Gap repair into the expression plasmid pTW438 |
5'-GGTTACCGCAAGTCCTCTTCAGAAATGAGCTTTTGCTCGGACGCC ATGGTGAGCTAAATATTCATAAGTCGACTACT |
| Q15A–F |
Site-directed mutagenesis |
5'-AGATTTTGATTTCGTTCTAGAAGCGGTTGGCAACTATGGTACTTA TCAGA |
| Q15A–R |
Site-directed mutagenesis |
5'-TCTGATAAGTACCATAGTTGCCAACCGCTTCTAGAACGAAATCA AAATCT |
| C31A–F |
Site-directed mutagenesis |
5'-ATTGTTTTCTTCTTTATAATTGCCCTTCCAACTAGTTTACCATCAG CATT |
| C31A–R |
Site-directed mutagenesis |
5'-AATGCTGATGGTAAACTAGTTGGAAGGGCAATTATAAAGAAGAA AACAAT |
| Q109A–F |
Site-directed mutagenesis |
5'-CGAATAAGTTTAGTGCCGTGCGCAAATGGATGGGATTATGATAA CTCTAC |
| Q109A–R |
Site-directed mutagenesis |
5'-GTAGAGTTATCATAATCCCATCCATTTGCGCACGGCACTAAACTT ATTCG |
| K300A–F |
Site-directed mutagenesis |
5'-CATCAACATCAACATTTGACGCTCCATTCATCTTTGCAATTTTCTT AAGC |
| K300A–R |
Site-directed mutagenesis |
5'-AAGAAAATTGCAAAGATGAATGGAGCGTCAAATGTTGATGTTG ATGAATT |
