Figure 4.

Aif-1 deficiency reduces CD4 T-cell expansion in the spleen. (A) Representative FACS data of effector T cells (CD3⁺CD4⁺; CD3⁺CD8⁺), B cells (CD3⁻B220⁺) and monocytes (CD45⁺CD11b⁺) from splenocytes of d-16 EAE wt and aif-1^−/− mice. (B) Absolute quantification of CD3⁺ splenocytes (left panel) and relative quantification of T-cell subsets, B cells and monocytes (right panel). (C, D) T-cell proliferation was measured by rechallenging spleno-cytes with α-CD3 antibodies and (D) MOG_35–55 to measure proliferation by [³H]thymidine in-corporation (n = 6). Data are represented as mean ± SEM. *P < 0.05; ****P < 0.0001.